Bioreactors For Animal Cell Culture

This article examines the underlying mechanisms involved in bubble associated cell damage and the methods available for controlling such damage.

Bioreactors for animal cell culture.

Home environment animal cell culture prev contents next bioreactors for suspension culture. Critical contamination points for a bioreactor are the transitions between sterile spaces such as the stirrer ports and gas lines. In general a conventional submerged type of bioreactor oxygenator with agitation system has been used in cell cultivations. Microcarriers with adherent cells which were in principle an adaptation of homogeneous culture systems used for.

4 1 1 and can be either mechanically driven stirred tank bioreactor or pneumatically driven bubble column air lift bioreactor. The biological systems involved include enzymes microorganisms animal cells plant cells and tissues. A bioreactor refers to any manufactured device or system that supports a biologically active environment. Animal cell culture bioreactors initially employed stirred tanks containing.

Bioreactor is defined as a vessel that carries out a biological reaction and is used to culture aerobic cells for conducting cellular or enzymatic immobilization. This process can either be aerobic or anaerobic these bioreactors are commonly cylindrical ranging in size from litres. Cell cultures are particularly susceptible to biological contamination. The same type of oxygenator as used for the homogeneous cultivation of microorganisms may be applied for plant cell cultivation and for micro carrier supported animal cell culture.

There are several types of bioreactors including stirred tank airlift hollow fiber and rotary cell culture system rccs d. The gas sparging of culture broth causes damage to suspended animal cells. Animal cell culture control for bioprocess engineering presents the design fabrication and control of a new type of bioreactor meant especially for animal cell line culture. Bioreactors play a key role in the field of biologics where they are used for the production of recombinant therapeutic proteins by large scale cultivation of animal cells.

Requirements for a bioreactor for animal cell culture 1 well controlled environment t ph do nutrients and wastes 2 supply of nutrients 3 gentle mixing avoid shear damage to cells 4 gentle aeration add oxygen slowly to the culture medium but avoid the formation of large bubbles which can damage cells on contact. One bacterial spore or vegetative bacterial cell is all it takes to overgrow an animal cell culture. Bioreactors for suspension culture belong to the group of dynamic systems compare sect. There are three major types of homogeneous oxygenator systems used for.

However despite this sparged bioreactors remain the preferred means of cell culture because sparging is a robust method of supplying oxygen especially on a large scale.